Sp6 rna polymerasemediated transcription试剂盒
WebIn vitro RNA synthesis with SP6 RNA polymerase. In vitro RNA synthesis with SP6 RNA polymerase Methods Enzymol. 1987;155:397-415. doi: 10.1016/0076-6879(87)55027-3. ... WebSP6 or T7 RNA polymerase promoter. This DNA produces a transcript approximately 1,800 bases long. Since luciferase must be full-length to show activity, transcription and translation of the Control DNA followed by a luciferase assay is a convenient means to verify that full-length transcripts have been generated.!
Sp6 rna polymerasemediated transcription试剂盒
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WebTRANSCRIPTION BY T7 AND SP6 RNA POLYMERASES VOL. 24, NO. 21, 1985 5717 FIGURE 1: Structure of pT7SP6 DNA. The two bacteriophage promoters are directed toward the same polylinker, but from opposite ... WebBacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. …
WebRNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions: 1X RNAPol Reaction Buffer, … Web30. jan 2015 · SP6 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from …
WebSP6 体外转录试剂盒 数量 25 次反应 促进剂 SP6 运输条件 干冰 产量 15-25 µg 原始材料 PCR 产物、线性化 DNA 质粒 内容与储存 SP6 酶混合物、10X 反应缓冲液、2XNTP⁄CAP 溶液 … WebT7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research. It can also produce highly sensitive radiolabeled RNA transcripts suitable for hybridization probes.
WebWe have constructed two sets of clones in which one to six head-to-tail connected DNA copies of the potato spindle tuber viroid (PSTV) RNA genome were inserted into the plasmid pSP62- Pl downstream of the promoter for SP6 RNA polymerase. In vitro transcription of these constructs with the promoter-s …
WebDocuments Thermo Scientific Bacteriophage SP6 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on … minecraft quark enchanting tableWebAssay Time: 145minutes Sample Materials Linearized plasmid DNA; PCR product ; Principle The DIG RNA Labeling Kit produces DIG-labeled, single-stranded RNA probes of known … morrowind tippsWeb碧云天生产的SP6 High Yield Transcription Kit，即SP6高产量RNA转录试剂盒，是一种利用SP6 RNA Polymerase能在相对较短时间内进行体外RNA大量转录的特性而研发的RNA体外 … minecraft quark githubWebHigh Quality & Proven Performance in RNA In Vitro Transcription Reactions Product Overview: SP6 RNA Polymerase is a recombinant protein from SP6 bacteriophage produced in E.coli. Highly specific for the SP6 promoter that makes it distinct from those of analogous enzymes. It catalyzes efficiently the synthesis of RNA from double-stranded DNA. morrowind tin whistleWebDocuments. This cloned high-purity Ambion™ SP6 RNA Polymerase has a high specificity for its promoter. The enzyme will transcribe large amounts of RNA from DNA sequences downstream of the promoter and show no … minecraft quark chuteWebSP6, T7 and T3 phage RNA polymerases have high specificity for their respective 23 base promoters (Figure 1). The development of cloning vectors containing promoters for these polymerases has made the in vitro synthesis of single stranded RNA molecules a routine laboratory procedure. morrowind tipsWebSP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and incorporates 35 S, 32 P and 33 P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone. SDS-polyacrylamide gel electrophoresis analysis … minecraft quark inventory