Phosphopeptide mapping
WebMar 19, 1999 · Comparative phosphopeptide mapping was carried out to characterize the phosphorylation status of inactive and active γ-PAK and to allow the identification of those phosphopeptides whose presence is indicative of γ-PAK activation. WebJan 1, 2002 · Phosphopeptides present in mixtures can be sequenced at the femtomole level without the need for extensive purification. A variety of mass spectrometry-based …
Phosphopeptide mapping
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WebApr 8, 2004 · Phosphopeptide mapping of wt Flag-eIF4B yielded a pattern identical to the endogenous eIF4B protein ( Figure 4C ). Mutation of Ser406 to Asp (not shown) or Ala ( Figure 4D) had no effect on the phosphopeptide pattern. However, mutation of Ser422 to Ala or Asp (not shown) eliminated phosphopeptide 2. WebDec 19, 1997 · Two-dimensional phosphopeptide mapping experiments indicate that Ser-831 is the major site of CaM kinase II phosphorylation on GluR1. In addition, treatment of hippocampal slice preparations with phorbol esters and forskolin increase the phosphorylation of serine 831 and 845, respectively, indicating that protein kinase C and …
WebSP, TP, and PxSP are common proline-directed kinase motifs, which function as substrates for MAP kinase, ERK1, and ERK2 (Flotow et al., 1990;Schwartz and Gygi, 2005; Yang and … WebJan 1, 2009 · In addition, phosphopeptide mapping can uncover the specific phosphorylated residue and hence, kinase recognition site, thus helping in the identification of the relevant …
WebJan 1, 2011 · The second approach is to determine the sites of phosphorylation of the receptor using mass spectrometry. This technique requires large (1 μg or more) amounts of purified receptor protein but has advantages over [32 P]-labelling and 2D phosphopeptide mapping in that the peptide identity and specific sites of phosphorylation can be … WebPhosphopeptide maps can also be used to find out whether the sites of phosphorylation on a protein change upon treatment of cells with certain agents. Procedures are also …
WebOct 1, 1998 · Mapping studies. Tryptic phosphopeptides from labelled Cdc25 were subjected to two-dimensional phosphopeptide mapping and reverse-phase HPLC 8 and …
WebAug 17, 2003 · In practice, this obligatory partial digestion is advantageous for phosphopeptide mapping because it provides staggered and redundant mass information for multiply phosphorylated peptides. the pavilion apartments macon gaWebJul 15, 2001 · The resulting phosphopeptide map reveals a single predominant spot (arrow) corresponding to the first LDSR motif in CPEB, and was identified by amino acid sequencing (Mendez et al., 2000a). A mix of the two tryptic digests (i.e. kinase sources were MI oocytes and recombinant Eg2) before the 2D mapping reveals co-migration of the LDSR ... shy excess all areas flac downloadWebFeb 2, 1999 · Phosphopeptide-specific antibodies confirm that this form of pRB has been phosphorylated on cdk4-specific, but not cdk2-specific, sites. Thus, p21 arrests cells by blocking the inactivation of pRB that normally occurs as cells progress through the G1phase of the cell cycle. the pavilion assiniboine parkWebJan 1, 2002 · Thus, it is possible to isolate rare proteins from large-scale cultures for phosphopeptide mapping studies. Regardless of the method used to map phosphorylation sites, it is imperative that the native phosphorylation state of the target protein be preserved during isolation. The chapter describes general strategies for isolating phosphoproteins ... the pavilion arts centre buxtonWebFeb 22, 2004 · Phosphopeptide mapping of the in vivo labeled vimentin shows that there is a large number of phosphorylation sites that undergo active turnover. As most of the phosphopeptides showing elevated phosphorylation in the presence of cl-A can also be discerned on the maps from control cells, we assume that the elevated phosphorylation is … shy fairy ornamentsWebFeb 2, 1999 · The two-dimensional phosphopeptide map of pRB from p21−/− cells was considerably more complex, containing more than 15 major phosphopeptides (labeled … the pavilion apartments charlottesville vaWebFeb 24, 2009 · To further confirm that the phosphorylation indeed occurred at the two proposed serine residues, we analyzed the His 6 -p53 bands from the kinase assay by phosphopeptide mapping. We detected the spot corresponding to serine 366 phosphorylation only in wild-type (WT) p53 samples, not in p53 AA samples ( Fig. 1 C ). shy face with keyboard