Iptg yeasen
WebYEASEN Precast Protein Plus Gel contains gradient concentration and fixed concentration, a variety of different concentrations can meet the needs of different protein electrophoresis. It is prepared by a unique gel preparation process and has an excellent electrophoretic effect. WebIPTG or Isopropyl β-D-1-thiogalactopyranoside is a molecular analog of allolactose, which removes a repressor from the lac operon to induce gene expression. Allolactose is an isomer of lactose, formed when lactose enters cells. It acts as an inducer to initiate the transcription of genes in the lac operon.
Iptg yeasen
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WebAfter 24 h of incubation, 4% paraformaldehyde was fixed and 0.5% Triton X-100 was permeabilized. Subsequently, they were sealed with bovine serum albumin (BSA). Finally, TRITC (Yeasen, Shanghai, China) was used to label the cytoskeleton and DAPI (Solarbio, Beijing, China) to label the nucleus. Images were taken under a fluorescence microscope ... Web热门搜索 : 细胞培养耗材，八联管，胎牛Serum，DMEM，胰酶，双抗，Tris，甘氨酸，离心管，吸头
WebInfobox references. Isopropyl β-d-1-thiogalactopyranoside ( IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator . WebOct 14, 2024 · When the optical density of the cultures reached 0.6–0.8, IPTG was added to a final concentration of 0.5 mM to induce protein expression. After 16 h of induction at 25 °C, the cells were harvested by centrifugation and lysed by sonification in a lysis buffer consisting of 10 mM Tris/HCl, pH 7.5, 150 mM NaCl and 20 mM imidazole.
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WebIPTG can't be used straight forward as an inducer in yeast because there is no natural lac promoter in yeast. However some recent data prove that IPTG regulated promoter may be …
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