WebSep 1, 2004 · The material was used within 18 h after preparation for inactivating the virus. ... Binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production. Arch Virol, 47 (1975), pp. 47-56. View in Scopus Google Scholar [3] F. Brown, J. Crick. WebThe reduced inactivation rates for binary ethylenimine directly prepared in the virus suspension are due to the different cyclization rates of 2-bromoethylamine hydrobromide and 2-chloroethylamine hydrochloride and to the interference of bicarbonate in the cyclization reaction.
Assessments of different inactivating reagents in formulating ...
WebWhen binary ethylenimine was prepared directly in the virus suspension only 2-bromoethylamine hydrobromide gave acceptable inactivation rates. The reduced … WebApr 7, 2024 · To inactivate the clarified viruses, 3 mM binary-ethylenimine (Sigma-Aldrich, St. Louis, MO, USA) was added, and the mixture was incubated in a shaking incubator for 24 h at 26 °C . The inactivated viruses were treated with a final concentration of 7.5% polyethylene glycol 6000 (Sigma-Aldrich) and 0.5 M NaCl (Sigma-Aldrich) for 16 h at 4 °C. chilly jilly\u0027z
Binary ethylenimine as an inactivant for foot-and-mouth disease …
WebJul 23, 2024 · The starting strains were obtained using ultraviolet (UV) irradiation and binary ethylenimine treatment followed by preparation of protoplasts, UV irradiation inactivation, multi-cell fusion, and fusion of the inactivated protoplasts to produce positive colonies. After two rounds of genome shuffling and the 96-PH method, six l-valine high ... WebJan 1, 2024 · Binary ethylenimine (BEI) has been widely used as a virucide to inactivate viruses. For regulatory exclusion of a select agent, the United States … WebAug 1, 1990 · The preparation and application of the aziridine compound binary ethylenimine (BEI) and the necessary conditions for and controls of the inactivation process are described and discussed. A computer program has been written for assistance in the use of BEI for controlled inactivation of viral antigens. grade 10 english curriculum